Hello there,
Welcome to the latest edition of the seqWell Scope!
This quarter we are highlighting plasmids, which are the bread and butter for many areas of life science research. These circular DNA molecules, which can be constructed with a diverse array of sequence features, have been a core tool of molecular biology for decades. Plasmids are the foundation for the new and evolving field of synthetic biology. In modern therapeutic areas, like gene editing with CRISPR-based technology, researchers use plasmids to create the DNA payloads that are then leveraged to engineer genomes and cells.
Utilizing plasmids for life sciences R&D often requires sequencing multiple synthetic constructs to identify clone sequences that have desired properties, or to confirm that the sequence of a synthesized DNA clone is correct for downstream applications. Plasmid sequencing has historically been performed with Sanger sequencing, but today’s generation of sequencing technologies can outperform this approach by enabling the sequencing of hundreds, even thousands, of individual clone sequences in a single run.
In this issue, we explore the many dimensions of high-throughput plasmid sequencing and focus on the innovations that are accelerating the adoption of modern sequencing platforms, such as Illumina-based sequencing in the area of high-throughput plasmid and synthetic clone sequencing. The articles and webinar below also highlight the powerful capabilities of seqWell’s ExpressPlex Library Prep Kits, the new paradigm of simplicity for large-scale workflows like plasmid and amplicon sequencing as well as advanced automation approaches for creating scalable workflows in synthetic biology.
I hope you enjoy this edition of the seqWell Scope, and if you would like to learn more please reach out to us any time. I also encourage you to connect with our team at the upcoming Festival of Genomics Boston (Booth #34) and American Society of Human Genetics 2023 Meeting in Washington, DC (Booth #1210).
